Thrust Area 2: Multi-Scale Thermodynamics of Water
Thrust Area 2 supports the ability to cryopreserve biological systems by preventing and/or mitigating ice crystallization of their water content during the cooling process even in systems as cold as -150 ºC, through strategic use of CPAs and manipulating the cooling conditions themselves. Three cryopreservation regimes are studied in ATP-Bio projects.
Thrust Area 2 overviews 15 projects involving researchers at MGH, UMN, UC Berkeley, TAMU, and UC Riverside of which many are interinstitutional. TA2 aims to reach success in the following milestones:
Controlling the phase change of water
Supercooling up to 2 L
Partial freezing and vitrification up to 100 mL
Isochoric biopreservation
Cryopreservation regimes
Outputs of the extreme value statistical model of
heterogeneous ice nucleation
Matthew Powell-Palm lab, TAMU
Cardiac MPS about to
undergo isochoric cooling
Kevin Healy lab, UC Berkeley
3D cryoprinter
Rubinsky lab, UC Berkeley
Rebecca Sandlin (MGH)
Thrust Area 2 Co-Lead
Boris Rubinsky (MGH)
Thrust Area 2 Co-Lead